Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562) at 1:1000 dilution or RPS6KA1 antibody (A15718). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-p90Rsk/RSK1/RPS6KA1-S380 pAb (AP0562). Western blot was performed from the immunoprecipitate using Phospho-p90Rsk/RSK1/RPS6KA1-S380 pAb (AP0562) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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Overview

Product name Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb
Catalog No. AP0562
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S380 of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
Sequence GFSFV
Gene ID 6195
Swiss prot Q15418
Synonyms RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A; Phospho-p90Rsk/RSK1/RPS6KA1-S380
Calculated MW 83kDa
Observed MW 90kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples HeLa, NIH/3T3
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb images

ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)}

Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562) at 1:1000 dilution or RPS6KA1 antibody (A15718). HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)}

Western blot - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)}

Immunoprecipitation - Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (AP0562)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-p90Rsk/RSK1/RPS6KA1-S380 pAb (AP0562). Western blot was performed from the immunoprecipitate using Phospho-p90Rsk/RSK1/RPS6KA1-S380 pAb (AP0562) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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Related Targets

Based on existing publications, the following genes are closely related to research on RPS6KA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RPS6KA1. (Distance between topics and target gene indicate popularity.) RPS6KA1

* Data provided by citexs.com, for reference only.

Publishing research using AP0562? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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