Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Publications (22) Datasheet

ABclonal:Phospho-NF-kB p65/RelA-S276 Rabbit pAb
ABclonal:Western blot - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Western blot analysis of extracts of various cell lines, using Phospho-NF-kB p65/RelA-S276 antibody (AP0123) at 1:1000 dilution.HeLa cells, NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunofluorescence analysis of U2OS cells using Phospho-NF-kB p65/RelA-S276 antibody (AP0123) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-NF-kB p65/RelA-S276 Rabbit pAb
Catalog No.AP0123
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S276 of human RelA (NP_068810.3).
SequenceRPSDR
Gene ID5970
Swiss protQ04206
SynonymsNFKB3; p65; NF-kB p65; RELA; CMCU
Calculated MW58kDa/59kDa/60kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, Schisandra chinensis)

IHC(Rattus norvegicus, Mus musculus)

IHC(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) }

Western blot - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Western blot analysis of extracts of various cell lines, using Phospho-NF-kB p65/RelA-S276 antibody (AP0123) at 1:1000 dilution.HeLa cells, NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123) }

Immunofluorescence - Phospho-NF-kB p65/RelA-S276 Rabbit pAb (AP0123)

Immunofluorescence analysis of U2OS cells using Phospho-NF-kB p65/RelA-S276 antibody (AP0123) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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