Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-NF2-S518 Rabbit pAb (AP1225)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-NF2-S518 Rabbit pAb (AP1225)

Western blot analysis of lysates from HeLa cells, using Phospho-NF2-S518 Rabbit pAb (AP1225) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name Phospho-NF2-S518 Rabbit pAb
Catalog No. AP1225
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S518 of human NF2 (NP_000259.1).
Sequence RLSME
Gene ID 4771
Swiss prot P35240
Synonyms ACN; SCH; BANF; merlin-1; Phospho-NF2-S518
Calculated MW 70kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane

Research Area

Phospho-NF2-S518 Rabbit pAb images

ABclonal:Western blot - Phospho-NF2-S518 Rabbit pAb (AP1225)}

Western blot - Phospho-NF2-S518 Rabbit pAb (AP1225)

Western blot analysis of lysates from HeLa cells, using Phospho-NF2-S518 Rabbit pAb (AP1225) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on NF2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NF2. (Distance between topics and target gene indicate popularity.) NF2

* Data provided by citexs.com, for reference only.

Publishing research using AP1225? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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