Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MyoD1-S200 Rabbit pAb (AP0150)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MyoD1-S200 Rabbit pAb (AP0150)

Western blot analysis of extracts from HeLa cells using Phospho-MyoD1-S200 antibody (AP0150).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MyoD1-S200 Rabbit pAb
Catalog No. AP0150
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S200 of human MYOD1
Sequence Email for sequence
Gene ID 4654
Swiss prot P15172
Synonyms PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF; Phospho-MyoD1-S200
Calculated MW 35kDa
Observed MW 40kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa
Cellular location Nucleus

Research Area

Phospho-MyoD1-S200 Rabbit pAb images

ABclonal:Western blot - Phospho-MyoD1-S200 Rabbit pAb (AP0150)}

Western blot - Phospho-MyoD1-S200 Rabbit pAb (AP0150)

Western blot analysis of extracts from HeLa cells using Phospho-MyoD1-S200 antibody (AP0150).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MYOD1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MYOD1. (Distance between topics and target gene indicate popularity.) MYOD1

* Data provided by citexs.com, for reference only.

Publishing research using AP0150? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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