Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Immunohistochemistry analysis of Phospho-c-Myc-S62 in paraffin-embedded rat testis using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Immunohistochemistry analysis of Phospho-c-Myc-S62 in paraffin-embedded human appendix using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Immunohistochemistry analysis of Phospho-c-Myc-S62 in paraffin-embedded mouse testis using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-c-Myc-S62 Rabbit pAb
Catalog No. AP0082
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S62 of human MYC (NP_001341799.1).
Sequence PLSPSR
Gene ID 4609
Swiss prot P01106
Synonyms MRTL; MYCC; c-Myc; bHLHe39; Phospho-c-Myc-S62
Calculated MW 51kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples NIH/3T3
Cellular location Nucleus, nucleolus, nucleoplasm

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-c-Myc-S62 Rabbit pAb images

ABclonal:Western blot - Phospho-c-Myc-S62 Rabbit pAb (AP0082)}

Western blot - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)}

Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Immunohistochemistry analysis of Phospho-c-Myc-S62 in paraffin-embedded rat testis using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)}

Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Immunohistochemistry analysis of Phospho-c-Myc-S62 in paraffin-embedded human appendix using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)}

Immunohistochemistry - Phospho-c-Myc-S62 Rabbit pAb (AP0082)

Immunohistochemistry analysis of Phospho-c-Myc-S62 in paraffin-embedded mouse testis using Phospho-c-Myc-S62 Rabbit pAb (AP0082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on MYC. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MYC. (Distance between topics and target gene indicate popularity.) MYC

* Data provided by citexs.com, for reference only.

Publishing research using AP0082? Please let us know so that we can cite the reference in this datasheet.

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* For research use only. Not for therapeutic or diagnostic purposes.

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