Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MET-Y1234/Y1235 Rabbit pAb (AP0408)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MET-Y1234/Y1235 Rabbit pAb (AP0408)

Western blot analysis of extracts from HUVEC cells, using Phospho-MET-Y1234/Y1235 antibody (AP0408).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MET-Y1234/Y1235 Rabbit pAb
Catalog No. AP0408
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding Y1234/Y1235 of human MET
Sequence Email for sequence
Gene ID 4233
Swiss prot P08581
Synonyms DA11; HGFR; AUTS9; RCCP2; c-Met; DFNB97; Phospho-MET-Y1234/Y1235
Calculated MW 156kDa
Observed MW 156kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HUVEC
Cellular location Membrane, Secreted, Single-pass type I membrane protein

Research Area

Phospho-MET-Y1234/Y1235 Rabbit pAb images

ABclonal:Western blot - Phospho-MET-Y1234/Y1235 Rabbit pAb (AP0408)}

Western blot - Phospho-MET-Y1234/Y1235 Rabbit pAb (AP0408)

Western blot analysis of extracts from HUVEC cells, using Phospho-MET-Y1234/Y1235 antibody (AP0408).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MET. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MET. (Distance between topics and target gene indicate popularity.) MET

* Data provided by citexs.com, for reference only.

Publishing research using AP0408? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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