Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MDM2-S166 Rabbit pAb (AP0073)

Reviews (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MDM2-S166 Rabbit pAb (AP0073)

Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S166 Rabbit pAb (AP0073). 293 cells were treated by Hydroxyurea (4mM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunofluorescence - Phospho-MDM2-S166 Rabbit pAb (AP0073)

Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using Phospho-MDM2-S166 Rabbit pAb (AP0073) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Review
ABclonal: review for Phospho-MDM2-S166 Rabbit pAb(AP0073)
Review
ABclonal: review for Phospho-MDM2-S166 Rabbit pAb(AP0073)

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Overview

Product name Phospho-MDM2-S166 Rabbit pAb
Catalog No. AP0073
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S166 of human MDM2 (NP_002383.2).
Sequence RAISET
Gene ID 4193
Swiss prot Q00987
Synonyms HDMX; LSKB; hdm2; ACTFS; Phospho-MDM2-S166
Calculated MW 55kDa
Observed MW 95kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples 293
Cellular location Cytoplasm, Nucleus, nucleolus, nucleoplasm
Customer validation

WB (Adenocarcinoma human alveolar basal epithelial cells)

IHC (Adenocarcinoma human alveolar basal epithelial cells)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-MDM2-S166 Rabbit pAb images

ABclonal:Western blot - Phospho-MDM2-S166 Rabbit pAb (AP0073)}

Western blot - Phospho-MDM2-S166 Rabbit pAb (AP0073)

Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S166 Rabbit pAb (AP0073). 293 cells were treated by Hydroxyurea (4mM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunofluorescence - Phospho-MDM2-S166 Rabbit pAb (AP0073)}

Immunofluorescence - Phospho-MDM2-S166 Rabbit pAb (AP0073)

Immunofluorescence analysis of paraffin-embedded Human breast cancer tissue using Phospho-MDM2-S166 Rabbit pAb (AP0073) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MDM2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MDM2. (Distance between topics and target gene indicate popularity.) MDM2

* Data provided by citexs.com, for reference only.

Publishing research using AP0073? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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