Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MAX-S11 Rabbit pAb (AP0072)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-MAX-S11 Rabbit pAb (AP0072)

Western blot analysis of lysates from Jurkat cells, using Phospho-MAX-S11 Rabbit pAb (AP0072) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MAX-S11 Rabbit pAb
Catalog No. AP0072
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S11 of human MAX (NP_002373.3).
Sequence VESDE
Gene ID 4149
Swiss prot P61244
Synonyms bHLHd4; Phospho-MAX-S11
Calculated MW 18kDa
Observed MW 21kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location Cell projection, Nucleus, dendrite

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-MAX-S11 Rabbit pAb images

ABclonal:Western blot - Phospho-MAX-S11 Rabbit pAb (AP0072)}

Western blot - Phospho-MAX-S11 Rabbit pAb (AP0072)

Western blot analysis of lysates from Jurkat cells, using Phospho-MAX-S11 Rabbit pAb (AP0072) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAX. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAX. (Distance between topics and target gene indicate popularity.) MAX

* Data provided by citexs.com, for reference only.

Publishing research using AP0072? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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