Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Mouse kidney using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Rat heart using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Human colon cancer using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Human lung cancer using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Rat brain using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Human placenta using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb
Catalog No. AP0396
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T334 of human MAPKAPK2 (NP_116584.2?).
Sequence PQTPL
Gene ID 9261
Swiss prot P49137
Synonyms MK2; MK-2; MAPKAP-K2; Phospho-MAPKAPK-2/MK2-T334
Calculated MW 46kDa
Observed MW

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Immunohistochemistry    
Positive samples
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb images

ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)}

Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Mouse kidney using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)}

Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Rat heart using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)}

Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Human colon cancer using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)}

Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Human lung cancer using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)}

Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Rat brain using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)}

Immunohistochemistry - Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396)

Immunohistochemistry analysis of Phospho-MAPKAPK-2/MK2-T334 in paraffin-embedded Human placenta using Phospho-MAPKAPK-2/MK2-T334 Rabbit pAb (AP0396) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPKAPK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPKAPK2. (Distance between topics and target gene indicate popularity.) MAPKAPK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0396? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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