Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MAPK9/MAPK10-T183 Rabbit pAb (AP0395)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MAPK9/MAPK10-T183 Rabbit pAb (AP0395)

Western blot analysis of extracts of 293 cells , using Phospho-MAPK9/MAPK10-T183 antibody (AP0395).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MAPK9/MAPK10-T183 Rabbit pAb
Catalog No. AP0395
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T183 of human MAPK9/MAPK10
Sequence Email for sequence
Gene ID 56015602
Swiss prot P45984P53779
Synonyms MAPK9/MAPK10; Phospho-MAPK9/MAPK10-T183
Calculated MW 27kDa/44kDa/48kDa/52kDa
Observed MW 54kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293, 293+Peptide
Cellular location cytoplasm, cytosol, mitochondrion, nuclear speck, nucleoplasm, nucleus, plasma membrane, Schaffer collateral - CA1 synapse

Phospho-MAPK9/MAPK10-T183 Rabbit pAb images

ABclonal:Western blot - Phospho-MAPK9/MAPK10-T183 Rabbit pAb (AP0395)}

Western blot - Phospho-MAPK9/MAPK10-T183 Rabbit pAb (AP0395)

Western blot analysis of extracts of 293 cells , using Phospho-MAPK9/MAPK10-T183 antibody (AP0395).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK9. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK9. (Distance between topics and target gene indicate popularity.) MAPK9

* Data provided by citexs.com, for reference only.

Publishing research using AP0395? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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