Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MAP3K5-S1033 Rabbit pAb (AP0058)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-MAP3K5-S1033 Rabbit pAb (AP0058)

Western blot analysis of lysates from A-431 and HeLa cells, using Phospho-MAP3K5-S1033 Rabbit pAb (AP0058) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MAP3K5-S1033 Rabbit pAb
Catalog No. AP0058
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S1033 of human MAP3K5 (NP_005914.1).
Sequence PPSPE
Gene ID 4217
Swiss prot Q99683
Synonyms ASK1; MEKK5; MAPKKK5; Phospho-MAP3K5-S1033
Calculated MW 155kDa
Observed MW 155kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A-431, HeLa
Cellular location Cytoplasm, Endoplasmic reticulum
Customer validation

WB (Mus musculus, Rattus norvegicus, Homo sapiens, Sus scrofa)

Documents

Certificate of Compliance

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Research Area

Phospho-MAP3K5-S1033 Rabbit pAb images

ABclonal:Western blot - Phospho-MAP3K5-S1033 Rabbit pAb (AP0058)}

Western blot - Phospho-MAP3K5-S1033 Rabbit pAb (AP0058)

Western blot analysis of lysates from A-431 and HeLa cells, using Phospho-MAP3K5-S1033 Rabbit pAb (AP0058) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAP3K5. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAP3K5. (Distance between topics and target gene indicate popularity.) MAP3K5

* Data provided by citexs.com, for reference only.

Publishing research using AP0058? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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