Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MEK3-S189 Rabbit pAb (AP0389)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - Phospho-MEK3-S189 Rabbit pAb (AP0389)

Western blot analysis of extracts from HeLa cells, using Phospho-MEK3-S189 antibody (AP0389).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-MEK3-S189 Rabbit pAb
Catalog No. AP0389
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S189 of human MEK3
Sequence Email for sequence
Gene ID 5606
Swiss prot P46734
Synonyms MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2; Phospho-MEK3-S189
Calculated MW 39kDa
Observed MW 39kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa
Cellular location cytosol, nucleoplasm

Research Area

Phospho-MEK3-S189 Rabbit pAb images

ABclonal:Western blot - Phospho-MEK3-S189 Rabbit pAb (AP0389)}

Western blot - Phospho-MEK3-S189 Rabbit pAb (AP0389)

Western blot analysis of extracts from HeLa cells, using Phospho-MEK3-S189 antibody (AP0389).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAP2K3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAP2K3. (Distance between topics and target gene indicate popularity.) MAP2K3

* Data provided by citexs.com, for reference only.

Publishing research using AP0389? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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