Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-MAP2K3-S189/MAP2K6-S207 Rabbit pAb (AP0066)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-MAP2K3-S189/MAP2K6-S207 Rabbit pAb (AP0066)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MAP2K3-S189/MAP2K6-S207 antibody (AP0066) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

You may also interested in:

Overview

Product name Phospho-MAP2K3-S189/MAP2K6-S207 Rabbit pAb
Catalog No. AP0066
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen A synthetic phosphorylated peptide around S189 of human MAP2K6MAP2K3 (NP_002747.2).
Sequence VDSVA
Gene ID 56065608
Swiss prot P46734P52564
Synonyms MAP2K3/MAP2K6; Phospho-MAP2K3-S189/MAP2K6-S207
Calculated MW 36kDa/39kDa/31kDa/37kDa
Observed MW 40kDa/41kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3
Cellular location cytosol, nucleoplasm

Research Area

Phospho-MAP2K3-S189/MAP2K6-S207 Rabbit pAb images

ABclonal:Western blot - Phospho-MAP2K3-S189/MAP2K6-S207 Rabbit pAb (AP0066)}

Western blot - Phospho-MAP2K3-S189/MAP2K6-S207 Rabbit pAb (AP0066)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-MAP2K3-S189/MAP2K6-S207 antibody (AP0066) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Inquire About This Product

Submit your question about AP0066 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on MAP2K3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAP2K3. (Distance between topics and target gene indicate popularity.) MAP2K3

* Data provided by citexs.com, for reference only.

Publishing research using AP0066? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order