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Phospho-LMNA-S22 pAb (AP0777)

Datasheet

ABclonal:Western blot - Phospho-LMNA-S22 pAb (AP0777)
ABclonal:Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777)

Overview

Product namePhospho-LMNA-S22 pAb
Catalog No.AP0777
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
ImmunogenA synthetic phosphorylated peptide around S22 of human LMNA (NP_005563.1).
SequencePLSPT
Gene ID4000
Swiss protP02545
SynonymsLMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin
Calculated MW62-74kDa
Observed MW69kDa, 78kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHeLa, C6
Cellular locationNucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
ABclonal:Western blot - Phospho-LMNA-S22 pAb (AP0777) }

Western blot - Phospho-LMNA-S22 pAb (AP0777)

Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody (AP0777) at 1:2000 dilution or LMNA antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.
ABclonal:Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777) }

Immunoprecipitation - Phospho-LMNA-S22 pAb (AP0777)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb (AP0777). Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb (AP0777) at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.

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