Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-LIMK2-T505 Rabbit pAb (AP0388)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-LIMK2-T505 Rabbit pAb (AP0388)

Western blot analysis of extracts from HeLa cells, using Phospho-LIMK2-T505 antibody (AP0388).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-LIMK2-T505 Rabbit pAb
Catalog No. AP0388
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding T505 of human LIMK2
Sequence Email for sequence
Gene ID 3985
Swiss prot P53671
Synonyms LIMK2; Phospho-LIMK2-T505
Calculated MW 72kDa
Observed MW 72kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:100 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa
Cellular location Cytoplasm, Nucleus, Nucleus

Research Area

Phospho-LIMK2-T505 Rabbit pAb images

ABclonal:Western blot - Phospho-LIMK2-T505 Rabbit pAb (AP0388)}

Western blot - Phospho-LIMK2-T505 Rabbit pAb (AP0388)

Western blot analysis of extracts from HeLa cells, using Phospho-LIMK2-T505 antibody (AP0388).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on LIMK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LIMK2. (Distance between topics and target gene indicate popularity.) LIMK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0388? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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