Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-LAT-Y171 Rabbit pAb (AP0809)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-LAT-Y171 Rabbit pAb (AP0809)

Western blot analysis of lysates from Jurkat cells, using Phospho-LAT-Y171 Rabbit pAb (AP0809) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

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Overview

Product name Phospho-LAT-Y171 Rabbit pAb
Catalog No. AP0809
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y171 of human LAT (NP_001014987.1).
Sequence DDYVN
Gene ID 27040
Swiss prot O43561
Synonyms LAT1; pp36; IMD52; Phospho-LAT-Y171
Calculated MW 28kDa
Observed MW 38kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location Cell membrane, Single-pass type III membrane protein

Research Area

Phospho-LAT-Y171 Rabbit pAb images

ABclonal:Western blot - Phospho-LAT-Y171 Rabbit pAb (AP0809)}

Western blot - Phospho-LAT-Y171 Rabbit pAb (AP0809)

Western blot analysis of lysates from Jurkat cells, using Phospho-LAT-Y171 Rabbit pAb (AP0809) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2mM) for 2 minutes after serum-starvation overnight or treated by CD-3 (10 μg/mL) for 2 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on LAT. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LAT. (Distance between topics and target gene indicate popularity.) LAT

* Data provided by citexs.com, for reference only.

Publishing research using AP0809? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (1)

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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