Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Jun-S243 Rabbit pAb (AP0050)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - Phospho-c-Jun-S243 Rabbit pAb (AP0050)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-c-Jun-S243 Rabbit pAb (AP0050) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-c-Jun-S243 Rabbit pAb (AP0050)

Immunohistochemistry analysis of paraffin-embedded rat lung using Phospho-c-Jun-S243 Rabbit pAb (AP0050) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-c-Jun-S243 Rabbit pAb
Catalog No. AP0050
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S243 of human Jun (NP_002219.1).
Sequence PLSPI
Gene ID 3725
Swiss prot P05412
Synonyms AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S243
Calculated MW 36kDa
Observed MW Refer to figures

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293, NIH/3T3
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-c-Jun-S243 Rabbit pAb images

ABclonal:Immunohistochemistry - Phospho-c-Jun-S243 Rabbit pAb (AP0050)}

Immunohistochemistry - Phospho-c-Jun-S243 Rabbit pAb (AP0050)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-c-Jun-S243 Rabbit pAb (AP0050) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-c-Jun-S243 Rabbit pAb (AP0050)}

Immunohistochemistry - Phospho-c-Jun-S243 Rabbit pAb (AP0050)

Immunohistochemistry analysis of paraffin-embedded rat lung using Phospho-c-Jun-S243 Rabbit pAb (AP0050) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on JUN. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to JUN. (Distance between topics and target gene indicate popularity.) JUN

* Data provided by citexs.com, for reference only.

Publishing research using AP0050? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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