Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Western blot analysis of extracts of UT7 cells, using Phospho-Jak2-Y1007/1008 antibody (AP0531).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

ABclonal:Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-Jak2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-Jak2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Jak2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).

Review
ABclonal: review for Phospho-Jak2-Y1007/1008 Rabbit pAb(AP0531)

Overview

Product namePhospho-Jak2-Y1007/1008 Rabbit pAb
Catalog No.AP0531
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.
ImmunogenA synthetic phosphorylated peptide around Y1007 & Y1008 of human Jak2 (NP_004963.1).
SequenceEYYK
Gene ID3717
Swiss protO60674
SynonymsJAK2; JTK10; THCYT3
Calculated MW130kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesUT7
Cellular locationCytoplasm, Endomembrane system, Nucleus, Peripheral membrane protein
Customer validation

(NCI-N87 cells)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531) }

Western blot - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Western blot analysis of extracts of UT7 cells, using Phospho-Jak2-Y1007/1008 antibody (AP0531).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
ABclonal:Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531) }

Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-Jak2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531) }

Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-Jak2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531) }

Immunohistochemistry - Phospho-Jak2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Jak2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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