|Product name||Phospho-JNK1/2-T183/Y185 Rabbit pAb|
|Purification method||Affinity purification|
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
|Immunogen||A synthetic phosphorylated peptide around T183 of human JNK1 (NP_620637.1).|
|Reactivity||Human, Mouse, Rat|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:2000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting |
|Positive samples||293, HeLa|
(Mouse embryonal fibroblast cell, Mouse heart tissue)
WB(Mus musculus, Homo sapiens, Rattus norvegicus)
Phospho-JNK1/2-T183/Y185 Rabbit pAb images
Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)
Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2-T183/Y185 antibody (AP0473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
* For research use only. Not for therapeutic or diagnostic purposes.
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