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Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)

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ABclonal:Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)

Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2-T183/Y185 antibody (AP0473) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Review
ABclonal: review for Phospho-JNK1/2-T183/Y185 Rabbit pAb(AP0473)
Review
ABclonal: review for Phospho-JNK1/2-T183/Y185 Rabbit pAb(AP0473)

Overview

Product namePhospho-JNK1/2-T183/Y185 Rabbit pAb
Catalog No.AP0473
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
ImmunogenA synthetic phosphorylated peptide around T183 of human JNK1 (NP_620637.1).
SequenceMMTPY
Gene ID5599/5601
Swiss protP45983/P45984
SynonymsJNK1/2
Calculated MW35kDa/44kDa/48kDa/27kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293, HeLa
Cellular location
Customer validation

(Mouse embryonal fibroblast cell, Mouse heart tissue)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

    ABclonal:Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473) }

    Western blot - Phospho-JNK1/2-T183/Y185 Rabbit pAb (AP0473)

    Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2-T183/Y185 antibody (AP0473) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.

    * For research use only. Not for therapeutic or diagnostic purposes.

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