Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Western blot analysis of extracts of HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at 1:3000 dilution.HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.293T cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.C6 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunohistochemistry analysis of paraffin-embedded rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)
Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631).NIH-3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.