Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Publications (5) Datasheet

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry of paraffin-embedded human appendix using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631).NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

Overview

Product namePhospho-JNK1/2/3-T183/T183/T221 Rabbit mAb
Catalog No.AP0631
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
ImmunogenA phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3
SequenceEmail for sequence
Gene ID5599/5601/5602
Swiss protP45983/P45984/P53779
SynonymsJNK1/JNK2/JNK3
Calculated MW35kDa/44kDa/48kDa/27kDa/52kDa
Observed MW46KDa/56KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, C6
Cellular location
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Mus musculus)

    ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) }

    Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) }

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) }

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry of paraffin-embedded human appendix using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) }

    Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) }

    Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

    Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631).NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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