Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Publications (63) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at 1:3000 dilution.HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.293T cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.C6 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631).NIH-3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

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Overview

Product name Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb
Catalog No. AP0631
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0193

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Immunogen information

Immunogen A synthetic phosphorylated peptide around T183 of human JNK1 (P45983).
Sequence MMTPY
Gene ID 559956015602
Swiss prot P45983P45984P53779
Synonyms JNK1/JNK2/JNK3; Phospho-JNK1/2/3-T183/T183/T221
Calculated MW 35kDa/44kDa/48kDa/27kDa/52kDa
Observed MW 46kDa/54kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanMouseRat
WB Human
IF/ICC Mouse
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa+Anisomycin, 293T+Anisomycin, 293T+UV, C6+UV, C6+Anisomycin
Cellular location axon, basal dendrite, cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus, synapse
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Capra hircus, Chrysanthemum, Bos taurus, Oryza sativa, Chlorocebus aethiops, Sus scrofa)

IHC (Mus musculus)

IF (Rattus norvegicus)

ICH (Chrysanthemum)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb images

ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of HeLa cells, using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631) at 1:3000 dilution.HeLa cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.293T cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of 293T, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Western blot - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Western blot analysis of extracts of C6, using Phospho-JNK1/2/3-T183/T183/T221 antibody (A23099) at 1:1000 dilution.C6 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 20 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunohistochemistry - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunohistochemistry analysis of paraffin-embedded rat liver using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)}

Immunofluorescence - Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (AP0631)

Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (AP0631).NIH-3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK8. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK8. (Distance between topics and target gene indicate popularity.) MAPK8

* Data provided by citexs.com, for reference only.

Publishing research using AP0631? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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