Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Review (1) Publications (33) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Western blot analysis of C6, using Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531) at 1:400 dilution.C6 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Phospho-JAK2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Phospho-JAK2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Phospho-JAK2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Review
ABclonal: review for Phospho-JAK2-Y1007/1008 Rabbit pAb(AP0531)

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Overview

Product name Phospho-JAK2-Y1007/1008 Rabbit pAb
Catalog No. AP0531
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a non-receptor tyrosine kinase that plays a central role in cytokine and growth factor signalling. The primary isoform of this protein has an N-terminal FERM domain that is required for erythropoietin receptor association, an SH2 domain that binds STAT transcription factors, a pseudokinase domain and a C-terminal tyrosine kinase domain. Cytokine binding induces autophosphorylation and activation of this kinase. This kinase then recruits and phosphorylates signal transducer and activator of transcription (STAT) proteins. Growth factors like TGF-beta 1 also induce phosphorylation and activation of this kinase and translocation of downstream STAT proteins to the nucleus where they influence gene transcription. Mutations in this gene are associated with numerous inflammatory diseases and malignancies. This gene is a downstream target of the pleiotropic cytokine IL6 that is produced by B cells, T cells, dendritic cells and macrophages to produce an immune response or inflammation. Disregulation of the IL6/JAK2/STAT3 signalling pathways produces increased cellular proliferation and myeloproliferative neoplasms of hematopoietic stem cells. A nonsynonymous mutation in the pseudokinase domain of this gene disrupts the domains inhibitory effect and results in constitutive tyrosine phosphorylation activity and hypersensitivity to cytokine signalling. This gene and the IL6/JAK2/STAT3 signalling pathway is a therapeutic target for the treatment of excessive inflammatory responses to viral infections. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y1007 & Y1008 of human JAK2 (NP_004963.1?).
Sequence KEYYKV
Gene ID 3717
Swiss prot O60674
Synonyms JTK10; Phospho-JAK2-Y1007/1008
Calculated MW 131kDa
Observed MW 125kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples C6+pervanadate
Cellular location Cytoplasm, Endomembrane system, Nucleus, Peripheral membrane protein
Customer validation

WB (NCI-N87 cellsHomo sapiens, Mus musculus, Homo sapiens, Rattus norvegicus)

IF (Mus musculus)

WB IHC (Mus musculus)

IHC (Gallus gallus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-JAK2-Y1007/1008 Rabbit pAb images

ABclonal:Western blot - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)}

Western blot - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Western blot analysis of C6, using Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531) at 1:400 dilution.C6 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)}

Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Phospho-JAK2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)}

Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Phospho-JAK2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)}

Immunohistochemistry - Phospho-JAK2-Y1007/1008 Rabbit pAb (AP0531)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Phospho-JAK2-Y1007/1008 antibody (AP0531) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on JAK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to JAK2. (Distance between topics and target gene indicate popularity.) JAK2

* Data provided by citexs.com, for reference only.

Publishing research using AP0531? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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