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Phospho-IRS1-S307 Rabbit pAb (AP0552)

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ABclonal:Western blot - Phospho-IRS1-S307 Rabbit pAb (AP0552)

Western blot analysis of extracts of mouse brain, using Phospho-IRS1-S307 antibody (AP0552) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for Phospho-IRS1-S307 Rabbit pAb(AP0552)

Overview

Product namePhospho-IRS1-S307 Rabbit pAb
Catalog No.AP0552
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
ImmunogenA synthetic phosphorylated peptide around S307 of human IRS1 (NP_005535.1).
SequenceTESIT
Gene ID3667
Swiss protP35568
SynonymsHIRS-1; IRS1
Calculated MW131kDa
Observed MW200kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse brian
Cellular location
Customer validation

(mouse stomach cells)

WB(Rattus norvegicus, Mus musculus)

ABclonal:Western blot - Phospho-IRS1-S307 Rabbit pAb (AP0552) }

Western blot - Phospho-IRS1-S307 Rabbit pAb (AP0552)

Western blot analysis of extracts of mouse brain, using Phospho-IRS1-S307 antibody (AP0552) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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