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Phospho-IRS1-S307 Rabbit pAb (AP0552)

Review (1)Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IRS1-S307 Rabbit pAb (AP0552)

Western blot analysis of extracts of various cell lines, using Phospho-IRS1-S307 antibody (AP0552) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10046 dilution.
Lysates/proteins: 71ug per lane.
Blocking buffer: 49% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00066).
Exposure time: 30s.

Review
ABclonal: review for Phospho-IRS1-S307 Rabbit pAb(AP0552)

Overview

Product namePhospho-IRS1-S307 Rabbit pAb
Catalog No.AP0552
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.
ImmunogenA synthetic phosphorylated peptide around S307 of human IRS1 (NP_005535.1).
SequenceTESIT
Gene ID3667
Swiss protP35568
SynonymsHIRS-1; IRS1
Calculated MW131kDa
Observed MW180KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, 293T
Cellular location
Customer validation

(mouse stomach cells)

WB(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - Phospho-IRS1-S307 Rabbit pAb (AP0552)}

Western blot - Phospho-IRS1-S307 Rabbit pAb (AP0552)

Western blot analysis of extracts of various cell lines, using Phospho-IRS1-S307 antibody (AP0552) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10046 dilution.
Lysates/proteins: 71ug per lane.
Blocking buffer: 49% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00066).
Exposure time: 30s.

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