Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-IRF3-S396 Rabbit pAb (AP0623)

Publications (3) Datasheet

ABclonal:Western blot - Phospho-IRF3-S396 Rabbit pAb (AP0623)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - Phospho-IRF3-S396 Rabbit pAb (AP0623)

Western blot analysis of extracts of 293T cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:500 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-IRF3-S396 Rabbit pAb
Catalog No.AP0623
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding S396 of human IRF3
SequenceEmail for sequence
Gene ID3661
Swiss protQ14653
SynonymsIIAE7; IRF3
Calculated MW12kDa/16kDa/33kDa/47kDa/49kDa
Observed MW45kDa/55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samples293T, NIH/3T3
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Sus scrofa)

ABclonal:Western blot - Phospho-IRF3-S396 Rabbit pAb (AP0623) }

Western blot - Phospho-IRF3-S396 Rabbit pAb (AP0623)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - Phospho-IRF3-S396 Rabbit pAb (AP0623) }

Western blot - Phospho-IRF3-S396 Rabbit pAb (AP0623)

Western blot analysis of extracts of 293T cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:500 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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