Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086) at 1:1000 dilution or IGF1R antibody (A0243).293T cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb
Catalog No. AP1086
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RINSR (NP_000199.2).
Sequence YETDYY
Gene ID 34803643
Swiss prot P08069P06213
Synonyms IGF1R/INSR; Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166
Calculated MW 154kDa/155kDa/156kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T
Cellular location cytoplasm, nucleus, plasma membrane, T-tubule
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb images

ABclonal:Western blot - Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)}

Western blot - Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086)

Western blot analysis of lysates from 293T cells, using Phospho-INSR-Y1189/Y1190 + IGF1R-Y1165/Y1166 Rabbit pAb (AP1086) at 1:1000 dilution or IGF1R antibody (A0243).293T cells were treated by IGF-1 (50 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on IGF1R. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IGF1R. (Distance between topics and target gene indicate popularity.) IGF1R

* Data provided by citexs.com, for reference only.

Publishing research using AP1086? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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