Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (AP0863)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (AP0863)

Western blot analysis of various lysates using Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (AP0863) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb
Catalog No. AP0863
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y1146 of human IGF1RINSR (NP_000199.2).
Sequence DIYET
Gene ID 36433480
Swiss prot P06213P08069
Synonyms INSR/IGF1R; Phospho-INSR-Y1146/IGF1R-Y1131
Calculated MW 155kDa
Observed MW 95kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T, HeLa
Cellular location Cell membrane, Single-pass type I membrane protein

Research Area

Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb images

ABclonal:Western blot - Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (AP0863)}

Western blot - Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (AP0863)

Western blot analysis of various lysates using Phospho-INSR-Y1146/IGF1R-Y1131 Rabbit pAb (AP0863) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on IGF1R. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IGF1R. (Distance between topics and target gene indicate popularity.) IGF1R

* Data provided by citexs.com, for reference only.

Publishing research using AP0863? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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