Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-IKKα/β-S176/180 Rabbit pAb (AP0546)

Publications (8) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IKKα/β-S176/180 Rabbit pAb (AP0546)

Western blot analysis of lysates from HeLa cells, using Phospho-IKKα/β-S176/180 Rabbit pAb (AP0546) at 1:1000 dilution.HeLa cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name Phospho-IKKα/β-S176/180 Rabbit pAb
Catalog No. AP0546
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen A synthetic phosphorylated peptide around S176 & S180 of human IKK-alphaCHUK (NP_001269.3).
Sequence SLCTS
Gene ID 11473551
Swiss prot O15111O14920
Synonyms
Calculated MW 84kDa/29kDa/79kDa/85kDa/86kDa
Observed MW 85kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa
Cellular location cytoplasm, cytoplasmic side of plasma membrane, cytosol, IkappaB kinase complex, nucleoplasm
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus)

Documents

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Lot number

Research Area

Phospho-IKKα/β-S176/180 Rabbit pAb images

ABclonal:Western blot - Phospho-IKKα/β-S176/180 Rabbit pAb (AP0546)}

Western blot - Phospho-IKKα/β-S176/180 Rabbit pAb (AP0546)

Western blot analysis of lysates from HeLa cells, using Phospho-IKKα/β-S176/180 Rabbit pAb (AP0546) at 1:1000 dilution.HeLa cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CHUK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CHUK. (Distance between topics and target gene indicate popularity.) CHUK

* Data provided by citexs.com, for reference only.

Publishing research using AP0546? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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