Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-IGF1R-Y1161 Rabbit pAb (AP0367)

Publications (5) Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IGF1R-Y1161 Rabbit pAb (AP0367)

Western blot analysis of lysates from 293 cells, using Phospho-IGF1R-Y1161 Rabbit pAb (AP0367).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-IGF1R-Y1161 Rabbit pAb
Catalog No. AP0367
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y1161 of human IGF1R (NP_000866.1).
Sequence DIYET
Gene ID 3480
Swiss prot P08069
Synonyms IGFR; CD221; IGFIR; JTK13; Phospho-IGF1R-Y1161
Calculated MW 155kDa
Observed MW 95kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293
Cellular location Cell membrane, Single-pass type I membrane protein
Customer validation

WB (Mus musculus, Homo sapiens)

IF (Mus musculus)

Research Area

Phospho-IGF1R-Y1161 Rabbit pAb images

ABclonal:Western blot - Phospho-IGF1R-Y1161 Rabbit pAb (AP0367)}

Western blot - Phospho-IGF1R-Y1161 Rabbit pAb (AP0367)

Western blot analysis of lysates from 293 cells, using Phospho-IGF1R-Y1161 Rabbit pAb (AP0367).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on IGF1R. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to IGF1R. (Distance between topics and target gene indicate popularity.) IGF1R

* Data provided by citexs.com, for reference only.

Publishing research using AP0367? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (1)

Proteins (3)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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