Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-IκBα-S36 Rabbit mAb (AP0999)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Western blot analysis of various lysates using Phospho-IκBα-S36 Rabbit mAb (AP0999) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight or treated by LPS (1 μg/mL) at 37℃ for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human colon carcinoma using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human colon using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human liver cancer using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human spleen using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human thyroid cancer using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded mouse intestin using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded mouse kidney using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded mouse liver using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-IκBα-S36 Rabbit mAb
Catalog No. AP0999
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC1543

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S36 of human IκBα (P25963).
Sequence LDSMK
Gene ID 4792
Swiss prot P25963
Synonyms IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S36
Calculated MW 36kDa
Observed MW 39kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanRat
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples THP-1+LPS, THP-1+PMA, NIH/3T3+TNF-α, C6+TNF-α
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

ELISA (Mus musculus)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-IκBα-S36 Rabbit mAb images

ABclonal:Western blot - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Western blot - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Western blot analysis of various lysates using Phospho-IκBα-S36 Rabbit mAb (AP0999) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight or treated by LPS (1 μg/mL) at 37℃ for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human colon carcinoma using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human colon using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human liver cancer using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human spleen using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded human thyroid cancer using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded mouse intestin using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded mouse kidney using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)}

Immunohistochemistry - Phospho-IκBα-S36 Rabbit mAb (AP0999)

Immunohistochemistry analysis of Phospho-IκBα-S36 in paraffin-embedded mouse liver using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on NFKBIA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NFKBIA. (Distance between topics and target gene indicate popularity.) NFKBIA

* Data provided by citexs.com, for reference only.

Publishing research using AP0999? Please let us know so that we can cite the reference in this datasheet.

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