Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-IκBα-S32 Rabbit mAb (AP0707)

Review (1) Publications (68) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IκBα-S32 Rabbit mAb (AP0707) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

Review
ABclonal: review for Phospho-IκBα-S32 Rabbit mAb(AP0707)

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Overview

Product name Phospho-IκBα-S32 Rabbit mAb
Catalog No. AP0707
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0147

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S32 of human IKB alpha (P25963).
Sequence HDSGL
Gene ID 4792
Swiss prot P25963
Synonyms IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S32
Calculated MW 36kDa
Observed MW 39kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB HumanMouseRat
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples NIH/3T3+TNF-α
Cellular location Cytoplasm, Nucleus
Customer validation

(A549)

WB (Mus musculus, Rattus norvegicus, Plant, Homo sapiens, Sus scrofa, Felis catus, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-IκBα-S32 Rabbit mAb images

ABclonal:Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)}

Western blot - Phospho-IκBα-S32 Rabbit mAb (AP0707)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-IκBα-S32 Rabbit mAb (AP0707) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

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Related Targets

Based on existing publications, the following genes are closely related to research on NFKBIA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NFKBIA. (Distance between topics and target gene indicate popularity.) NFKBIA

* Data provided by citexs.com, for reference only.

Publishing research using AP0707? Please let us know so that we can cite the reference in this datasheet.

Antibodies (14)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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