Western blot analysis of extracts of HeLa and 293 cells, using Phospho-IκBα-S32/36 antibody (AP0614) at 1:1000 dilution. HeLa cells were treated by TNF-α (20ng/mL) for 30 minutes. 293 cells were treated by TNF-α (20ng/mL) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA
|Product name||Phospho-IκBα-S32/36 Rabbit pAb|
|Purification method||Affinity purification|
|Tested applications||WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Recommended dilution||WB 1:500 - 1:1000|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Positive samples||HeLa, 293|
|Cellular location||Cytoplasm, Nucleus|
WB(Mus musculus, Homo sapiens, Gallus gallus, Cavia porcellus, Rattus norvegicus, Schisandra chinensis)
* For research use only. Not for therapeutic or diagnostic purposes.
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