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Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Publications (15) Datasheet

ABclonal:Western blot - Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Western blot analysis of extracts of HeLa and 293 cells, using Phospho-IκBα-S32/36 antibody (AP0614) at 1:1000 dilution. HeLa cells were treated by TNF-α (20ng/mL) for 30 minutes. 293 cells were treated by TNF-α (20ng/mL) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA

Overview

Product namePhospho-IκBα-S32/36 Rabbit pAb
Catalog No.AP0614
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
ImmunogenA synthetic phosphorylated peptide around S32 & S36 of human IκBα (NP_065390.1).
SequenceSGLDS
Gene ID4792
Swiss protP25963
SynonymsIKBA; MAD-3; NFKBI; IKB alpha; NFKBIA; EDAID2
Calculated MW35kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, 293
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens, Gallus gallus, Cavia porcellus, Rattus norvegicus, Schisandra chinensis)

ABclonal:Western blot - Phospho-IκBα-S32/36 Rabbit pAb (AP0614) }

Western blot - Phospho-IκBα-S32/36 Rabbit pAb (AP0614)

Western blot analysis of extracts of HeLa and 293 cells, using Phospho-IκBα-S32/36 antibody (AP0614) at 1:1000 dilution. HeLa cells were treated by TNF-α (20ng/mL) for 30 minutes. 293 cells were treated by TNF-α (20ng/mL) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA

* For research use only. Not for therapeutic or diagnostic purposes.

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