Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of lysates from C6 cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Immunofluorescence analysis of H9C2 cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Immunofluorescence analysis of U2OS cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.

You may also interested in:

Overview

Product name Phospho-Histone H3-S28 Rabbit pAb
Catalog No. AP0839
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S28 of human Histone H3 (NP_003520.1).
Sequence ARKSA
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Phospho-Histone H3-S28
Calculated MW 16kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, NIH/3T3
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Phospho-Histone H3-S28 Rabbit pAb images

ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of lysates from C6 cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution.C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Western blot - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at 1:900 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
ABclonal:Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Immunofluorescence analysis of H9C2 cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)}

Immunofluorescence - Phospho-Histone H3-S28 Rabbit pAb (AP0839)

Immunofluorescence analysis of U2OS cells using Phospho-Histone H3-S28 Rabbit pAb (AP0839) at dilution of 1:100. Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about AP0839 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on H3C1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3C1. (Distance between topics and target gene indicate popularity.) H3C1

* Data provided by citexs.com, for reference only.

Publishing research using AP0839? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order