Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Histone H3-S10 Rabbit mAb (AP0002)

PathoQIHC Pathology

Review (1) Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Western blot analysis of lysates from HeLa, NIH/3T3, C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at 1:1000 dilution. HeLa, NIH/3T3 and C6 cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.

ABclonal:Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded human oophoroma using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded mouse kidney using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of HeLa cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Review
ABclonal: review for Phospho-Histone H3-S10 Rabbit mAb(AP0002)

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Overview

Product name Phospho-Histone H3-S10 Rabbit mAb
Catalog No. AP0002
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0003

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S10 of human Histone H3 (P68431).
Sequence ARKST
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Phospho-Histone H3-S10
Calculated MW 15kDa
Observed MW 15kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications Testing results
IHC-P Human
WB HumanMouseRat
IF/ICC MouseRat
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6
Cellular location Chromosome, Nucleus
Customer validation

(HEK293T cells)

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Phospho-Histone H3-S10 Rabbit mAb images

ABclonal:Western blot - Phospho-Histone H3-S10 Rabbit mAb (AP0002)}

Western blot - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Western blot analysis of lysates from HeLa, NIH/3T3, C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at 1:1000 dilution. HeLa, NIH/3T3 and C6 cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 1s.
ABclonal:Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)}

Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded human oophoroma using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)}

Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Immunohistochemistry analysis of Phospho-Histone H3-S10 in paraffin-embedded mouse kidney using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)}

Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)}

Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of HeLa cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)}

Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3C1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3C1. (Distance between topics and target gene indicate popularity.) H3C1

* Data provided by citexs.com, for reference only.

Publishing research using AP0002? Please let us know so that we can cite the reference in this datasheet.

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