Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Reviews (3)Publications (16) Datasheet

ABclonal:Phospho-Histone H2AX-S139 Rabbit pAb
ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (AP0099) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of extracts of C6 cells, using Phospho-Histone H2AX-S139 antibody (AP0099) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of C6 cells using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of HeLa cells using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.HeLa cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit pAb(AP0099)
Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit pAb(AP0099)
Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit pAb(AP0099)

Overview

Product namePhospho-Histone H2AX-S139 Rabbit pAb
Catalog No.AP0099
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
ImmunogenA synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).
SequenceTQASQ
Gene ID3014
Swiss protP16104
SynonymsH2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; histone H2AX; gamma H2A.X; γH2AX
Calculated MW15kDa
Observed MW15KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

(U251 cells, N2A, Mouse bone marrow)

IF(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

WB(Homo sapiens, Mus musculus)

ChIP

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (AP0099) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Western blot - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of extracts of C6 cells, using Phospho-Histone H2AX-S139 antibody (AP0099) at 1:500 dilution.C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of C6 cells using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of HeLa cells using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.HeLa cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099) }

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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