Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Review (1) Publications (28) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (AP0687) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Western blot analysis of extracts of 293T cells, using Phospho-Histone H2AX-S139 antibody (AP0687) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry analysis of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of NIH-3T3 (treated with UV, 30 mJ/cm2) and NIH/3T3 (untreated), using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-Histone H2AX-S139 Rabbit mAb(AP0687)

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Overview

Product name Phospho-Histone H2AX-S139 Rabbit mAb
Catalog No. AP0687
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0110

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S139 of human Histone H2A.X (P16104).
Sequence TQASQ
Gene ID 3014
Swiss prot P16104
Synonyms H2A.X; H2A/X; H2AFX; Phospho-Histone H2AX-S139
Calculated MW 15kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB HumanMouseRat
IHC-P MouseRat
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T+UV, NIH/3T3+UV
Cellular location Chromosome, Nucleus
Customer validation

(HUH7)

IHC (Mus musculus, Homo sapiens)

WB (Homo sapiens, Mus musculus, Sus scrofa)

IF (Mus musculus, Homo sapiens, Sus scrofa, Macaca fascicularis)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-Histone H2AX-S139 Rabbit mAb images

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)}

Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (AP0687) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)}

Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Western blot analysis of extracts of 293T cells, using Phospho-Histone H2AX-S139 antibody (AP0687) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry analysis of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)}

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)}

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of NIH-3T3 (treated with UV, 30 mJ/cm2) and NIH/3T3 (untreated), using Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H2AX. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H2AX. (Distance between topics and target gene indicate popularity.) H2AX

* Data provided by citexs.com, for reference only.

Publishing research using AP0687? Please let us know so that we can cite the reference in this datasheet.

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