Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)

Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040) at 1:600 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunoprecipitation - Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-HSP27/HSPB1-S15 pAb (AP0040). Western blot was performed from the immunoprecipitate using Phospho-HSP27/HSPB1-S15 pAb (AP0040) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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Overview

Product name Phospho-HSP27/HSPB1-S15 Rabbit pAb
Catalog No. AP0040
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S15 of human HSP27/HSPB1 (NP_001531.1).
Sequence GPSWD
Gene ID 3315
Swiss prot P04792
Synonyms CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; Phospho-HSP27/HSPB1-S15
Calculated MW 23kDa
Observed MW 27kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples HeLa, HeLa
Cellular location Cytoplasm, Nucleus, cytoskeleton, spindle
Customer validation

WB (Mesocricetus auratus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-HSP27/HSPB1-S15 Rabbit pAb images

ABclonal:Western blot - Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)}

Western blot - Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)

Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040) at 1:600 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunoprecipitation - Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)}

Immunoprecipitation - Phospho-HSP27/HSPB1-S15 Rabbit pAb (AP0040)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-HSP27/HSPB1-S15 pAb (AP0040). Western blot was performed from the immunoprecipitate using Phospho-HSP27/HSPB1-S15 pAb (AP0040) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.

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Related Targets

Based on existing publications, the following genes are closely related to research on HSPB1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSPB1. (Distance between topics and target gene indicate popularity.) HSPB1

* Data provided by citexs.com, for reference only.

Publishing research using AP0040? Please let us know so that we can cite the reference in this datasheet.

Antibodies (10)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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