Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-HSF1-S307 Rabbit pAb (AP0364)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-HSF1-S307 Rabbit pAb (AP0364)

Western blot analysis of extracts from HUVEC cells, using Phospho-HSF1-S307 antibody (AP0364).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-HSF1-S307 Rabbit pAb
Catalog No. AP0364
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S307 of human HSF1
Sequence Email for sequence
Gene ID 3297
Swiss prot Q00613
Synonyms HSTF1; Phospho-HSF1-S307
Calculated MW 57kDa
Observed MW 80kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HUVEC
Cellular location Cytoplasm, Nucleus

Research Area

Phospho-HSF1-S307 Rabbit pAb images

ABclonal:Western blot - Phospho-HSF1-S307 Rabbit pAb (AP0364)}

Western blot - Phospho-HSF1-S307 Rabbit pAb (AP0364)

Western blot analysis of extracts from HUVEC cells, using Phospho-HSF1-S307 antibody (AP0364).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on HSF1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HSF1. (Distance between topics and target gene indicate popularity.) HSF1

* Data provided by citexs.com, for reference only.

Publishing research using AP0364? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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