Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)

Western blot analysis of lysates from HeLa cells, using Phospho-HP1 gamma/CBX3-S83 pAb (AP0801) at 1:1000 dilution or HP1 gamma/CBX3 antibody (A2248).HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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Overview

Product name Phospho-HP1 gamma/CBX3-S83 Rabbit pAb
Catalog No. AP0801
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S83 of human HP1 gamma/CBX3 (NP_009207.2).
Sequence RKSLS
Gene ID 11335
Swiss prot Q13185
Synonyms HECH; HP1-GAMMA; HP1Hs-gamma; Phospho-HP1 gamma/CBX3-S83
Calculated MW 21kDa
Observed MW 22kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A-431, NIH/3T3
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-HP1 gamma/CBX3-S83 Rabbit pAb images

ABclonal:Western blot - Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)}

Western blot - Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)

Western blot analysis of lysates from HeLa cells, using Phospho-HP1 gamma/CBX3-S83 pAb (AP0801) at 1:1000 dilution or HP1 gamma/CBX3 antibody (A2248).HeLa cells were treated by Paclitaxel (100 nM/mL) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)}

Western blot - Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HP1 gamma/CBX3-S83 Rabbit pAb (AP0801) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on CBX3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CBX3. (Distance between topics and target gene indicate popularity.) CBX3

* Data provided by citexs.com, for reference only.

Publishing research using AP0801? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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