Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-H2AX-S139 Rabbit pAb (AP0099)

Reviews (3)Publications (9) Datasheet

ABclonal:Western blot - Phospho-H2AX-S139 Rabbit pAb (AP0099)
ABclonal:Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099)
ABclonal:Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099)
ABclonal:Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099)
ABclonal:Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099)
Review
ABclonal: review for Phospho-H2AX-S139 Rabbit pAb(AP0099)
Review
ABclonal: review for Phospho-H2AX-S139 Rabbit pAb(AP0099)
Review
ABclonal: review for Phospho-H2AX-S139 Rabbit pAb(AP0099)

Overview

Product namePhospho-H2AX-S139 Rabbit pAb
Catalog No.AP0099
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
ImmunogenA synthetic phosphorylated peptide around S139 of human H2AX (NP_002096.1).
SequenceTQASQ
Gene ID3014
Swiss protP16104
SynonymsH2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; histone H2AX; gamma H2A.X; γH2AX
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

(U251 cells, N2A, Mouse bone marrow)

IF(Homo sapiens)

ChIP

WB(Mus musculus, Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Western blot - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Western blot analysis of extracts of various cell lines, using Phospho-Histone H2AX-S139 antibody (AP0099) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) for 5 hours. NIH/3T3 cells were treated by UV for 15-30 minutes. C6 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Immunohistochemistry - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody (AP0099) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of C6 cells using Phospho-Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of HeLa cells using Phospho-Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.HeLa cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099) }

Immunofluorescence - Phospho-H2AX-S139 Rabbit pAb (AP0099)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-Histone H2AX-S139 antibody (AP0099) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by UV for 15-30 minutes at RT (left). Blue: DAPI for nuclear staining.

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