Products > Catalog Antibodies > Phosphorylated Antibodies

Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Publication (1) Datasheet

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (AP0687) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry of paraffin-embedded human esophageal using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of C6 cells using Phospho-Histone H2AX-S139 antibody (AP0687). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of HeLa cells using Phospho-Histone H2AX-S139 antibody (AP0687). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H2AX-S139 antibody (AP0687). Blue: DAPI for nuclear staining.

Overview

Product namePhospho-Histone H2AX-S139 Rabbit mAb
Catalog No.AP0687
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
ImmunogenA synthesized peptide derived from human Phospho-Histone H2A.X (S139).
SequenceEmail for sequence
Gene ID3014
Swiss protP16104
SynonymsH2A.X; H2A/X; H2AX; Histone H2AX; H2AFX; histone H2AX; gamma H2A.X; γH2AX
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3
Cellular locationChromosome, Nucleus
Customer validation

IHC(Mus musculus)

ABclonal:Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Western blot - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (AP0687) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry of paraffin-embedded human esophageal using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Immunohistochemistry - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody (AP0687) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of C6 cells using Phospho-Histone H2AX-S139 antibody (AP0687). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of HeLa cells using Phospho-Histone H2AX-S139 antibody (AP0687). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687) }

Immunofluorescence - Phospho-Histone H2AX-S139 Rabbit mAb (AP0687)

Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H2AX-S139 antibody (AP0687). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about AP0687 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AP0687? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order