Reviews (3)Publications (13) Datasheet
Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat
Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 antibody (AP0039) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 pAb (AP0039) at 1:2000 dilution or GSK3β antibody (A2081).HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Phospho-GSK3β-S9 antibody (AP0039). Western blot was performed from the immunoprecipitate using Phospho-GSK3β-S9 antibody (AP0039) at a dilition of 1:1000.
| Product name | Phospho-GSK3β-S9 Rabbit pAb |
|---|---|
| Catalog No. | AP0039 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | HeLa |
| Cellular location | Cell membrane, Cytoplasm, Nucleus |
| Customer validation | (rat cells, Mouse N2a,Mouse kidney) WB(Rattus norvegicus, Homo sapiens, Mus musculus) IHC(Rattus norvegicus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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