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Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Reviews (3)Publications (11) Datasheet

ABclonal:Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 antibody (AP0039) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 pAb (AP0039) at 1:2000 dilution or GSK3β antibody (A2081).HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Phospho-GSK3β-S9 antibody (AP0039). Western blot was performed from the immunoprecipitate using Phospho-GSK3β-S9 antibody (AP0039) at a dilition of 1:1000.

Review
ABclonal: review for Phospho-GSK3β-S9 Rabbit pAb(AP0039)
Review
ABclonal: review for Phospho-GSK3β-S9 Rabbit pAb(AP0039)
Review
ABclonal: review for Phospho-GSK3β-S9 Rabbit pAb(AP0039)

Overview

Product namePhospho-GSK3β-S9 Rabbit pAb
Catalog No.AP0039
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S9 of human GSK3β (NP_001139628.1).
SequenceTTSFA
Gene ID2932
Swiss protP49841
SynonymsGSK3B; gsk-3β
Calculated MW46kDa/48kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

(rat cells, Mouse N2a,Mouse kidney)

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Rattus norvegicus)

ABclonal:Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039) }

Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 antibody (AP0039) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039) }

Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Western blot analysis of extracts of HeLa cells, using Phospho-GSK3β-S9 pAb (AP0039) at 1:2000 dilution or GSK3β antibody (A2081).HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039) }

Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039) }

Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Phospho-GSK3β-S9 Rabbit pAb (AP0039) }

Immunoprecipitation - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Phospho-GSK3β-S9 antibody (AP0039). Western blot was performed from the immunoprecipitate using Phospho-GSK3β-S9 antibody (AP0039) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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