Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Reviews (3) Publications (32) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Western blot analysis of lysates from C6 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunohistochemistry analysis of Phospho-GSK3β-S9 in paraffin-embedded human colon tissue using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunohistochemistry analysis of Phospho-GSK3β-S9 in paraffin-embedded human colon carcinoma tissue using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunohistochemistry analysis of Phospho-GSK3β-S9 in paraffin-embedded human liver cancer tissue using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb(AP0039) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb(AP0039) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of HeLa CA and HeLa cells using Phospho-GSK3β-S9 Rabbit pAb(AP0039) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Phospho-GSK3β-S9 Rabbit pAb(AP0039)
Review
ABclonal: review for Phospho-GSK3β-S9 Rabbit pAb(AP0039)
Review
ABclonal: review for Phospho-GSK3β-S9 Rabbit pAb(AP0039)

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Overview

Product name Phospho-GSK3β-S9 Rabbit pAb
Catalog No. AP0039
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S9 of human GSK3β (NP_001139628.1).
Sequence TTSFAE
Gene ID 2932
Swiss prot P49841
Synonyms GSK3B; gsk-3β; Phospho-GSK3β-S9
Calculated MW 47kDa
Observed MW 46 kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples C6
Cellular location Cell membrane, Cytoplasm, Nucleus
Customer validation

WB (rat cells, Mouse N2a,Mouse kidneyRattus norvegicus, Homo sapiens, Mus musculus, Rattus norvegicus, Crassostrea gigas, Gallus gallus)

IHC (Mouse N2a,Mouse kidneyRattus norvegicus)

CO-IP (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-GSK3β-S9 Rabbit pAb images

ABclonal:Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Western blot - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Western blot analysis of lysates from C6 cells using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunohistochemistry analysis of Phospho-GSK3β-S9 in paraffin-embedded human colon tissue using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunohistochemistry analysis of Phospho-GSK3β-S9 in paraffin-embedded human colon carcinoma tissue using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Immunohistochemistry - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunohistochemistry analysis of Phospho-GSK3β-S9 in paraffin-embedded human liver cancer tissue using Phospho-GSK3β-S9 Rabbit pAb (AP0039) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb(AP0039) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb(AP0039) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)}

Immunofluorescence - Phospho-GSK3β-S9 Rabbit pAb (AP0039)

Immunofluorescence analysis of HeLa CA and HeLa cells using Phospho-GSK3β-S9 Rabbit pAb(AP0039) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on GSK3B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GSK3B. (Distance between topics and target gene indicate popularity.) GSK3B

* Data provided by citexs.com, for reference only.

Publishing research using AP0039? Please let us know so that we can cite the reference in this datasheet.

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