Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Publications (11) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261) at 1:1000 dilution. NIH/3T3 cells were treated by λPP (20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Immunohistochemistry analysis of Phospho-GSK3β-Y216 + GSK3α-Y279 in paraffin-embedded mouse testis tissue using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Immunohistochemistry analysis of Phospho-GSK3β-Y216 + GSK3α-Y279 in paraffin-embedded mouse kidney tissue using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Overview

Product name Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb
Catalog No. AP0261
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y216/Y279 of human GSK3β/GSK3α(NP_001139628/NP_063937.2).
Sequence VSYIC
Gene ID 29312932
Swiss prot P49841
Synonyms GSK3B; gsk-3β; Phospho-GSK3β-Y216 + GSK3α-Y279
Calculated MW 47kDa
Observed MW 47kDa, 51kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples NIH/3T3
Cellular location Cell membrane, Cytoplasm, Nucleus
Customer validation

WB (Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus)

IF (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb images

ABclonal:Western blot - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)}

Western blot - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261) at 1:1000 dilution. NIH/3T3 cells were treated by λPP (20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)}

Immunohistochemistry - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Immunohistochemistry analysis of Phospho-GSK3β-Y216 + GSK3α-Y279 in paraffin-embedded mouse testis tissue using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)}

Immunohistochemistry - Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261)

Immunohistochemistry analysis of Phospho-GSK3β-Y216 + GSK3α-Y279 in paraffin-embedded mouse kidney tissue using Phospho-GSK3β-Y216 + GSK3α-Y279 Rabbit pAb (AP0261) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GSK3B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GSK3B. (Distance between topics and target gene indicate popularity.) GSK3B

* Data provided by citexs.com, for reference only.

Publishing research using AP0261? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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