Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-GluR1/GRIA1-S849 Rabbit pAb (AP0242)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-GluR1/GRIA1-S849 Rabbit pAb (AP0242)

Western blot analysis of extracts from rat brain tissue using Phospho-GluR1/GRIA1-S849 antibody (AP0242).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-GluR1/GRIA1-S849 Rabbit pAb
Catalog No. AP0242
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A phospho specific peptide corresponding to residues surrounding S849 of human GluR1/GRIA1
Sequence Email for sequence
Gene ID 2890
Swiss prot P42261
Synonyms GLUH1; GLUR1; GLURA; GluA1; HBGR1; MRD67; MRT76; Phospho-GluR1/GRIA1-S849
Calculated MW 102kDa
Observed MW 102kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Rat brain
Cellular location Cell junction, Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, dendrite, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse

Research Area

Phospho-GluR1/GRIA1-S849 Rabbit pAb images

ABclonal:Western blot - Phospho-GluR1/GRIA1-S849 Rabbit pAb (AP0242)}

Western blot - Phospho-GluR1/GRIA1-S849 Rabbit pAb (AP0242)

Western blot analysis of extracts from rat brain tissue using Phospho-GluR1/GRIA1-S849 antibody (AP0242).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on GRIA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GRIA1. (Distance between topics and target gene indicate popularity.) GRIA1

* Data provided by citexs.com, for reference only.

Publishing research using AP0242? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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