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Phospho-GRIA1-S845 pAb (AP0825)

Datasheet

ABclonal:Western blot - Phospho-GRIA1-S845 pAb (AP0825)

Overview

Product namePhospho-GRIA1-S845 pAb
Catalog No.AP0825
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA phospho specific peptide corresponding to residues surrounding S845 of human GRIA1
SequenceEmail for sequence
Gene ID2890
Swiss protP42261
SynonymsGRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1; glutamate receptor 1
Calculated MW92kDa/93kDa/101kDa/102kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesSH-SY5Y
Cellular locationCell junction, Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, dendrite, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse
ABclonal:Western blot - Phospho-GRIA1-S845 pAb (AP0825) }

Western blot - Phospho-GRIA1-S845 pAb (AP0825)

Western blot analysis of extracts of SH-SY5Y cells, using Phospho-GRIA1-S845 antibody (AP0825) at 1:1000 dilution or GRIA1 antibody (A1826). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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