Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-Connexin 43-S368 Rabbit pAb (AP0355)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Connexin 43-S368 Rabbit pAb (AP0355)

Western blot analysis of extracts of various cell lines, using Phospho-Connexin 43-S368 antibody (AP0355) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Phospho-Connexin 43-S368 Rabbit pAb (AP0355)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma using Phospho-Connexin 43-S368 antibody (AP0355).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-Connexin 43-S368 Rabbit pAb
Catalog No. AP0355
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S368 of human GJA1 (NP_000156.1).
Sequence RASSR
Gene ID 2697
Swiss prot P17302
Synonyms HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA; Phospho-Connexin 43-S368
Calculated MW 43kDa
Observed MW 35kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, NIH/3T3
Cellular location Cell junction, Cell membrane, Endoplasmic reticulum, Multi-pass membrane protein, gap junction

Research Area

Phospho-Connexin 43-S368 Rabbit pAb images

ABclonal:Western blot - Phospho-Connexin 43-S368 Rabbit pAb (AP0355)}

Western blot - Phospho-Connexin 43-S368 Rabbit pAb (AP0355)

Western blot analysis of extracts of various cell lines, using Phospho-Connexin 43-S368 antibody (AP0355) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Phospho-Connexin 43-S368 Rabbit pAb (AP0355)}

Immunohistochemistry - Phospho-Connexin 43-S368 Rabbit pAb (AP0355)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma using Phospho-Connexin 43-S368 antibody (AP0355).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on GJA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GJA1. (Distance between topics and target gene indicate popularity.) GJA1

* Data provided by citexs.com, for reference only.

Publishing research using AP0355? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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