Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Western blot analysis of various lysates using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at 1:500 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Overview

Product name Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb
Catalog No. AP0892
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]

Immunogen information

Immunogen A synthetic phosphorylated peptide around T24 of human FOXO1/FOXO3A/FOXO4FOXO1 (NP_002006.2).
Sequence SCTWP
Gene ID 230823094303
Swiss prot Q12778O43524
Synonyms FoxO1/FoxO3a/FoxO4; Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28
Calculated MW
Observed MW 65kDa/73kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Jurkat, C6
Cellular location cytoplasm, cytosol, mitochondrion, nucleoplasm, nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb images

ABclonal:Western blot - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)}

Western blot - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Western blot analysis of various lysates using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at 1:500 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)}

Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded rat liver using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)}

Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded human liver cancer using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)}

Immunohistochemistry - Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892)

Immunohistochemistry analysis of Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 in paraffin-embedded mouse kidney using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (AP0892) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on FOXO1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FOXO1. (Distance between topics and target gene indicate popularity.) FOXO1

* Data provided by citexs.com, for reference only.

Publishing research using AP0892? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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