Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-c-Fos-T232 Rabbit pAb (AP0038)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-c-Fos-T232 Rabbit pAb (AP0038)

Western blot analysis of lysates from HepG2 cells, using Phospho-c-Fos-T232 Rabbit pAb (AP0038) at 1:1000 dilution or c-Fos antibody (A17351).HepG2 cells were treated by 10% FBS for after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Overview

Product name Phospho-c-Fos-T232 Rabbit pAb
Catalog No. AP0038
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

Immunogen information

Immunogen A synthetic phosphorylated peptide around T232 of human c-Fos (NP_005243.1).
Sequence VATPE
Gene ID 2353
Swiss prot P01100
Synonyms p55; AP-1; C-FOS; Phospho-c-Fos-T232
Calculated MW 41kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HepG2
Cellular location Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Customer validation

WB (Chlorocebus aethiops, Gallus gallus, Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-c-Fos-T232 Rabbit pAb images

ABclonal:Western blot - Phospho-c-Fos-T232 Rabbit pAb (AP0038)}

Western blot - Phospho-c-Fos-T232 Rabbit pAb (AP0038)

Western blot analysis of lysates from HepG2 cells, using Phospho-c-Fos-T232 Rabbit pAb (AP0038) at 1:1000 dilution or c-Fos antibody (A17351).HepG2 cells were treated by 10% FBS for after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

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Related Targets

Based on existing publications, the following genes are closely related to research on FOS. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FOS. (Distance between topics and target gene indicate popularity.) FOS

* Data provided by citexs.com, for reference only.

Publishing research using AP0038? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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