Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-FLT3-Y589/591 Rabbit pAb (AP0529)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-FLT3-Y589/591 Rabbit pAb (AP0529)

Western blot analysis of lysates from A-431 cells, using Phospho-FLT3-Y589/591 Rabbit pAb (AP0529) at 1:500 dilution.A-431 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name Phospho-FLT3-Y589/591 Rabbit pAb
Catalog No. AP0529
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y589 & Y591 of human FLT3 (NP_004110.2).
Sequence EYFYV
Gene ID 2322
Swiss prot P36888
Synonyms FLK2; STK1; CD135; FLK-2; Phospho-FLT3-Y589/591
Calculated MW 113kDa
Observed MW 160kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples A-431
Cellular location Endoplasmic reticulum lumen, Membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

Phospho-FLT3-Y589/591 Rabbit pAb images

ABclonal:Western blot - Phospho-FLT3-Y589/591 Rabbit pAb (AP0529)}

Western blot - Phospho-FLT3-Y589/591 Rabbit pAb (AP0529)

Western blot analysis of lysates from A-431 cells, using Phospho-FLT3-Y589/591 Rabbit pAb (AP0529) at 1:500 dilution.A-431 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on FLT3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FLT3. (Distance between topics and target gene indicate popularity.) FLT3

* Data provided by citexs.com, for reference only.

Publishing research using AP0529? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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