Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-EphA3-Y779 Rabbit pAb (AP1056)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-EphA3-Y779 Rabbit pAb (AP1056)

Western blot analysis of lysates from 293T cells, using Phospho-EphA3-Y779 Rabbit pAb (A8414).293T cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Overview

Product name Phospho-EphA3-Y779 Rabbit pAb
Catalog No. AP1056
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Immunogen information

Immunogen A synthetic phosphorylated peptide around Y779 of human EphA3 (NP_005224.2).
Sequence AAYTT
Gene ID 2042
Swiss prot P29320
Synonyms EK4; ETK; HEK; ETK1; HEK4; TYRO4; Phospho-EphA3-Y779
Calculated MW 110kDa
Observed MW 110kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 293T
Cellular location Cell membrane, Secreted, Single-pass type I membrane protein

Research Area

Phospho-EphA3-Y779 Rabbit pAb images

ABclonal:Western blot - Phospho-EphA3-Y779 Rabbit pAb (AP1056)}

Western blot - Phospho-EphA3-Y779 Rabbit pAb (AP1056)

Western blot analysis of lysates from 293T cells, using Phospho-EphA3-Y779 Rabbit pAb (A8414).293T cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on EPHA3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EPHA3. (Distance between topics and target gene indicate popularity.) EPHA3

* Data provided by citexs.com, for reference only.

Publishing research using AP1056? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

Proteins (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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