Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP1120)

Publications (6) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP1120)

Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP1120) at 1:1000 dilution or ERK1 / ERK2 antibody (A16686).Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb
Catalog No. AP1120
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

Immunogen information

Immunogen A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
Sequence LTEYV
Gene ID 5594 5595
Swiss prot P28482P27361
Synonyms
Calculated MW
Observed MW 42kDa/44kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples
Cellular location caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane
Customer validation

WB (floral, Rattus norvegicus, Bos taurus, Homo sapiens)

Documents

Certificate of Compliance

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Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb images

ABclonal:Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP1120)}

Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP1120)

Western blot analysis of lysates from Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP1120) at 1:1000 dilution or ERK1 / ERK2 antibody (A16686).Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on MAPK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to MAPK1. (Distance between topics and target gene indicate popularity.) MAPK1

* Data provided by citexs.com, for reference only.

Publishing research using AP1120? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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